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Expression, purification and crystallization of wheat profilin (Tri a 12)
Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coli, purified and crystallized using the hangingdrop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 Å. The crystals belonged to space group P3221, with unit-cell parameters a = b = ...
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ژورنال
عنوان ژورنال: Open Journal of Immunology
سال: 2016
ISSN: 2162-450X,2162-4526
DOI: 10.4236/oji.2016.64017